Effect of miR223 on production of IL-1beta and IL-6 in gingival epithelial cells

Inhalt:
The aims of this research are exploring the expression of miR223 when the Porphyromonas gingivalis lipopolysaccharide (Pg. LPS) stimulated human gingival epithelial cells (HGECs); and investigating the effects of miR223 on the levels of interleukin-1beta (IL-1beta) and IL-6 in Pg. LPS-induced HGECs inflammation responses. We established the cell model of periodontitis by Pg. LPS stimulation of HGECs with different concentrations and time. The miR223 was detected by real-time PCR. To determine the effects of miR-223 on the production of IL-1beta and IL-6, HGECs were transfected with a miR-223 expression plasmid and stimulated by Pg. LPS (10mg/L). Levels of NLRP3 mRNA in HEGCs were also investigated by real-time PCR. The expression of IL-1beta and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA). The obtained results showed that miR-223 expression is dramatically reduced in HGECs by Pg. LPS stimulation. The down-regulated miR-223 leads to the increase in the NLRP3 expression and a promotion of IL-6 and IL-1beta production upon Pg. LPS stimulation. However, increased miR-223 expression obviously in HGECs, which in turn suppressed NLRP3 expression and downstream IL-1beta, IL-6 production. Our findings suggest that miR223 might attenuate the inflammatory responses in periodontal tissues.