MKL-1 enhances luciferase activity of a reporter gene containing the miR-142 promoter in MDA-MB-231 cells

Abstract:
Breast cancer is a malignant tumor that occurs in the mammary epithelial tissue, miR-142 was reported to abnormally expressed in breast cancer tissues with BRCA1/2 mutation, However, the definite transcription mechanism of miR-142 gene is unclear. In this study, human miR-142 promoter luciferase reporter construct was generated by PCR amplification. Then the PCR fragment was digested and cloned into pGL3 vector, and this promoter sequence was verified by sequencing. The result showed that luciferase reporter with miR-142 promoter was successfully constructed. Then the activation of the miR-142 promoter was detected in MDA-MB-231 cells by luciferase reporter assay after transfected expression vectors of human MKL-1. The result showed that transfected the expression vectors can enhance the transcriptional activity of miR-142. Our research will reveal the effect of miR-142 on breast cancer and further reveal the proliferation and differentiation of breast cancer cells and may provide theoretical foundations for methods to cure breast cancer.