Bovine Oocyte Characterization by Microfluidic Aspiration-Assisted Electrical Impedance Spectroscopy
Conference: MikroSystemTechnik Kongress 2023 - Kongress
10/23/2023 - 10/25/2023 at Dresden, Deutschland
Proceedings: MikroSystemTechnik Kongress 2023
Pages: 3Language: englishTyp: PDF
Authors:
Cao, Yuan; Schnakenberg, Uwe (Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Aachen, Germany)
Fries, Ann-Selina; Granacher, Olav; Wrenzycki, Christine (Animal Clinic for Reproductive Medicine and Neonatology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Germany)
Floehr, Julia (Helmholtz-Institute for Biomedical Engineering, Uniklinik RWTH Aachen University, Aachen, Germany)
Abstract:
This work presents a microfluidic aspiration-assisted electrical impedance spectroscopy system (MAEIS) to characterize bovine oocytes during a standard in vitro maturation (IVM) procedure. The hardening of the zona pellucida, which is a gelatinous outer layer of extracellular matrix, was characterized by measuring the electrical impedance at oocytes which were hydroynamically trapped at a microhole at a stable aspiration pressure. After EIS measurements, DNA staining was applied to determine the germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase II (MII) stages of nuclear maturation. The data showed that the impedance of bovine oocytes increased from GV to GVBD and decreased in the following MI and MII stages indicating that the maturation status of the bovine oocytes can be distinguished via EIS measurement.