Dynamic fluorescence measurements on DNA microarrays in foil-based microfluidics using multichannel Silicon Photomultipliers.
Conference: MikroSystemTechnik 2017 - Kongress
10/23/2017 - 10/25/2017 at München, Deutschland
Proceedings: MikroSystemTechnik 2017
Pages: 4Language: englishTyp: PDF
Personal VDE Members are entitled to a 10% discount on this title
Authors:
Ohlander, Anna; Seb, Andrej (Fraunhofer EMFT, Hansastr. 27d, 80686 München, Germany)
Bose, Indranil; Kutter, Christoph (Fraunhofer EMFT, Hansastr. 27d, 80686 München, Germany & Institute of Physics, Universität der Bundeswehr 85577 München, Germany)
Ganka, Thomas; Wiest, Florian (Ketek GmbH, Hoferstr. 3, 81476, München, Germany)
Abstract:
We report on multiplexed detection of SNPs (Single Nucleotide Polymorphisms) in a low-cost foil-based microfluidic detection module using small multichannel Silicon Photomultiplier detectors (SiPM). The microfluidic module uses integrated DNA microarrays and thin-film microheaters to enable surface-bound melting curve analysis (MCA) for SNP scoring.